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Topoisomerase I deficiency results inchromosomal alterations in cervical cancer cells

Kjeldsen, Eigil, Tordrup, David, Hubner, Gerda M., Knudsen, Birgitta R. and Andersen, Felicie F. (2010) Topoisomerase I deficiency results inchromosomal alterations in cervical cancer cells. Anticancer Research, 30. pp. 3257-3266. ISSN 0250-7005

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Human topoisomerase I has been suggested to be implicated in the maintenance of genomic stability via its ability to regulate genome topology during transcription and replication. In the present study, we demonstrate by wholegenome array comparative genomic hybridization (aCGH) and fluorescence in situ hybridisation (FISH) analysis that topoisomerase I deficiency results in chromosome 5p gain in the cervical cancer cell line, HeLa-CCL2. In contrast, chromosome 5p copy number remained unaffected by topoisomerase I downregulation in the non-cancer cell line HEK293T, as demonstrated by FISH analysis. Chromosome 5p gain is the most frequent genetic alteration in invasive cervical cancer, which leads to overexpression of genes involved in proliferation and occurs primarily at late stages in cancer development. The amplification of this region upon topoisomerase I downregulation specifically in HeLa-CCL2 cells may indicate an important role of topoisomerase I in preventing malignant progression of precancerous lesions in the cervix.

Item Type: Article
Official URL:
Additional Information: © 2010 by The International Institute of Anticancer Research
Divisions: LSE Health
Subjects: R Medicine > R Medicine (General)
Date Deposited: 27 Jun 2013 08:26
Last Modified: 16 May 2024 01:10

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